Publications 2017-12-19T22:50:06+00:00

Publications

Services

Names of ILS employees are shown in boldface font:

Proceedings of the 2018 National Toxicology Program Satellite Symposium

October 8, 2018

Susan A. Elmore, Vinicius Carreira, Caralyn S. Labriola, Debabrata Mahapatra, Sean R. McKeag, Matthias Rinke, Cynthia Shackelford, Bhanu Singh, Ashley Talley, Shannon M. Wallace, Lyn M. Wancket, Cynthia J. Willson

Differentiating between Testicular Toxicity and Sexual Immaturity in Ortho-phthalaldehyde Inhalation Toxicity Studies in Rats and Mice

October 8, 2018

 

Natasha R. Catlin, Cynthia J. Willson, Dianne M. Creasy, Deepa B. Rao, Grace E. Kissling, Barry S. McIntyre, Michael Wyde

Confirmation of high-throughput screening data and novel mechanistic insights into VDR-xenobiotic interactions by orthogonal assays

June 11, 2018

Debabrata Mahapatra, Jill A. Franzosa, Kyle Roell, Melaine Agnes Kuenemann, Keith A. Houck, David M. Reif, Denis Fourches & Seth W. Kullman

Genotoxicity evaluation of the naturally-derived food colorant, gardenia blue, and its precursor, genipin

June 4, 2018

Cheryl Hobbs, Mihoko Koyanagi, Carol Swartz, Jeffrey Davis, Robert Maronpot, Leslie Recio, Shim-mo Hayashi

Carcinogenic activity of pentabrominated diphenyl ether mixture (DE-71) in rats and mice

May 14, 2018

 

J.K. Dunnick, A.R. Pandiri, B.A. Merrick, G.E. Kissling, H. Cunny, E. Mutlu, S. Waidyanatha, R. Sills, H.L. Hong, TV. Ton, T. Maynor, L. Recio, S.L. Phillips, M.J. Devito, A. Brix

Role of pathology peer review in interpretation of the comet assay

May 11, 2018

R. R. Maronpot, Cheryl A. Hobbs, Shim-mo Hayashi

Black cohosh extracts and powders induce micronuclei, a biomarker of genetic damage, in human cells

April 25, 2018

Stephanie L. Smith‐Roe, Carol D. Swartz, Kim G. Shepard, Steven M. Bryce, Stephen D. Dertinger, Suramya Waidyanatha, Grace E. Kissling, Scott S. Auerbach, Kristine L. Witt, E. Zeiger

Transcriptional profiling of male CD-1 mouse lungs and harderian glands supports the involvement of calcium signaling in acrylamide-induced tumors

February 26, 2018

Nikolai L. Chepelev⁠a, Rémi Gagné⁠, Timothy Maynor⁠, Byron Kuo⁠, Cheryl A. Hobbs⁠, Leslie Recio⁠, Carole L. Yauk⁠

Magnesium stearate, a widely-used food additive, exhibits a lack of in vitro and in vivo genotoxic potential

February 26, 2018

Cheryl A. Hobbs, Kazuhiko Saigo, Mihoko Koyanagi, Shim-mo Hayashi

Comprehensive evaluation of the flavonol anti-oxidants, alpha-glycosyl isoquercitrin and isoquercitrin, for genotoxic potential

February 23, 2018

Cheryl A. Hobbs, Mihoko Koyanagi, Carol Swartz, Jeffrey Davis, Sawako Kasamoto, Robert Maronpot, Leslie Recio, Shim-mo Hayashi

Comparative inhalation toxicity of ethyltoluene isomers in rats and mice

January 29, 2018

Georgia K. Roberts, Cynthia J. Willson, Dorian S. Olivera, David E. Malarkey &
Daniel L. Morgan

In Vitro to In Vivo extrapolation for high throughput prioritization and decision making

January 3, 2018

 

Bell SM, Chang X, Wambaugh JF, Allen DG, Bartels M, Brouwer KLR, Casey WM, Choksi N, Ferguson SS, Fraczkiewicz G, Jarabek AM, Ke A, Lumen A, Lynn SG, Paini A, Price PS, Ring C, Simon TW, Sipes N, Sprankle CS, Strickland J, Troutman J, Wetmore BA, Kleinstreuer NC. 2017. In vitro to in vivo extrapolation for high throughput prioritization and decision making. Toxicology In Vitro. 2017 Dec 5. doi:10.1016/j.tiv.2017.11.016

Open-source Workflows for In Vitro to In Vivo Extrapolation

March 16, 2017

X Chang, SM Bell, JR Pirone, D Allen, W Casey, N Kleinstreuer. 2017. Open-source Workflows for In Vitro to In Vivo Extrapolation. Presented at SOT Annual Meeting on March 16, 2017 (Abstract # 3185 )

Development of a Curated Database of In Vivo Developmental Toxicity Data

March 16, 2017

P Ceger, N Choksi, J Hamm, J Truax, A Daniel, D Allen, V Sutherland, B McIntyre, P Foster, E Maull, W Casey. 2017. Development of a Curated Database of In Vivo Developmental Toxicity Data. Presented at SOT Annual Meeting on March 16, 2017 (Abstract #3159)

Impact of Acrylamide on Calcium Signaling and Cytoskeletal Filaments in Testes From F344 Rat.

March 16, 2017

 

Leslie Recio, Marvin Friedman, Dennis Marroni, Timothy Maynor, Nikolai Chepelev

An Integrated Chemical Environment to Support 21st Century Toxicology

March 15, 2017

SM Bell, J Phillips, A Sedykh, C Sprankle, S Morefield, D Allen, W Casey, N Kleinstreuer. 2017. An Integrated Chemical Environment to Support 21st Century Toxicology. Presented at SOT Annual Meeting on March 15, 2017 (Abstract # 2935)

Prediction of Skin Sensitization Potency Using Machine Learning Approaches

March 15, 2017

J Strickland, Q Zang, M Paris, DM Lehmann, S Bell, D Allen, J Matheson, A Jacobs, W Casey, N Kleinstreuer. 2017. Prediction of Skin Sensitization Potency Using Machine Learning Approaches. Presented at SOT Annual Meeting on March 15, 2017 (Abstract #2915)

Identification, Curation, and Prioritization of Food-Use Chemicals in ToxCast

March 15, 2017

M Krishan, TD Trautman, DF Kapraun, LA Fix, and AL Karmaus. 2017. Identification, Curation, and Prioritization of Food-Use Chemicals in ToxCast. Presented at SOT Annual Meeting on March 15, 2017 (Abstract #2559)

In Vitro Micronucleus and CometChip with Metabolically Competent HepaRG Cells

March 14, 2017

 

Swartz, CD, Winters, J, Owens, K, Yauk, CL, Buick, J, Ngo, L, Engelward, B, and Recio, L. 2017. In Vitro Micronucleus® and CometChip with Metabolically Competent HepaRG™ Cells. Presented at SOT Annual Meeting on March 14, 2017 (Abstract # 2267)

Acrylamide Perturbs Calcium and Actin Cytoskeleton Signaling in Rat and Mouse Cancer Target Tissues

March 14, 2017

Chepelev NL, Gagné R, Maynor T, Hobbs CA, Kuo B, Yauk CL, Recio L. 2017. Acrylamide Perturbs Calcium and Actin Cytoskeleton Signaling in Rat and Mouse Cancer Target Tissues. Presented at SOT Annual Meeting on March 14, 2017 (Abstract # 1830)

Customizing the Connectivity Map Approach for Functional Evaluation in Toxicogenomics Studies

March 13, 2017

Karmaus AL, Kothiya P, Watford S, Thomas RS, Martin MT. 2017. Customizing the Connectivity Map Approach for Functional Evaluation in Toxicogenomics Studies. Presented at SOT Annual Meeting on March 13, 2017 (Abstract #1374)

Toxicogenomics Reveals Dynamic Baseline Changes and Overlapping Effects of Epigenotoxicant Exposure on Stem Cell Differentiation and Tissue Development Networks

March 13, 2017

Hobbs CA, Sinha N, Chepelev N, Gagné R, Christy N, Yauk CL, Recio L. 2017. Toxicogenomics Reveals Dynamic Baseline Changes and Overlapping Effects of Epigenotoxicant Exposure on Stem Cell Differentiation and Tissue Development Networks. Presented at SOT Annual Meeting on March 13, 2017 (Abstract #1344)

Ninety-day toxicity and single-dose toxicokinetics study of AGIQ in Sprague-Dawley rats

September 27, 2016

Nyska A, Hayashi S, Koyanagi M, Davis J, Jokinen M, Ramot Y, Maronpot R. 2016. Ninety-day toxicity and single-dose toxicokinetics study of alphaglycosyl isoquercitrin in Sprague-Dawley rats. Food and Chemical Toxicology. 2016 Sep 27. pii: S0278-6915(16)30346-5.

Genotoxicity assessment of the flavouring agent, perillaldehyde

September 1, 2016

Hobbs C, Taylor S, Beevers C, Lloyd M, Bowen R, Lillford L, Maronpot R, Hayashi S. 2016. Genotoxicity assessment of the flavouring agent, perillaldehyde. Food and Chemical Toxicology. 2016 Sep 1;97:232-242. doi: 10.1016/j.fct.2016.08.029.

Chemical Reactivity and Respiratory Toxicity of the alpha-Diketone Flavoring Agents

July 1, 2016

Morgan D, Jokinen M, Johnson C, Price H, Gwinn W, Bousquet R, Flake G. 2016. Chemical Reactivity and Respiratory Toxicity of the alpha-Diketone Flavoring Agents: 2,3-Butanedione, 2,3-Pentanedione, and 2,3-Hexanedione. Toxicologic Pathology. 2016 Jul;44(5):763-83. doi: 10.1177/0192623316638962.

Differential genotoxicity of acrylamide in the MN and Pig-a assays in F344 rats and B6C3F1 mice

June 23, 2016

Hobbs CA, Davis J, Shepard K, Chepelev N, Friedman M, Marroni D, Recio L. 2016. Differential genotoxicity of acrylamide in the micronucleus and Pig-a gene mutation assays in F344 rats and B6C3F1 mice. Mutagenesis. 2016 Jun 23. pii: gew028

Advancing toxicology research using in vivo high throughput toxicology with small fish models

June 21, 2016

Planchart A, Mattingly CJ, Allen D, Ceger P., Casey W, Hinton D, Kanungo J, Kullman SW, Tal T, Bondesson M, Burgess SM, Sullivan C, Kim C, Behl M, Padilla S, Reif DM, Tanquay RL, Hamm J. 2016. Advancing toxicology research using in vivo high throughput toxicology with small fish models. Altex. 2016 Jun 21. doi: 10.14573/altex.1601281.

Integrated decision strategies for skin sensitization hazard

March 14, 2016

Strickland J, Zang Q, Kleinstreuer N, Paris M, Lehmann DM, Choksi N, Matheson J, Jacobs A, Lowit A, Allen D, Casey W. 2016. Integrated decision strategies for skin sensitization hazard. Journal of Applied Toxicology. 2016 Feb 6. doi: 10.1002/jat.3281.

Accelerating adverse outcome pathway development using publicly available data sources

March 14, 2016

Oki NO, Nelms MD, Bell SM, Mortensen HM, Edwards SW. 2016. Accelerating adverse outcome pathway development using publicly available data sources. Curr Environ Health Rep. 2016 Mar;3(1):53-63.

Adverse Outcome Pathways: From Research to Regulation. Scientific workshop report

March 14, 2016

Kleinstreuer NC, Sullivan K, Allen D, Edwards S, Mendrick DL, Embry M, Matheson J, Rowlands JC, Munn S, Maull E, Casey W. 2016. Adverse Outcome Pathways: From Research to Regulation. Scientific workshop report. Regulatory Toxicology & Pharmacology. 2016 Jan 14;76:39-50 doi: 10.1016/j.yrtph.2016.01.007

Enhanced stability of TTP mRNA protects mice against immune-mediated inflammatory pathologies

February 8, 2016

Patial S, Curtis AD, Lai WS, Stumpo DJ, Hill GD, Flake GP, Mannie MD, Blackshear PJ. Enhanced stability of tristetraprolin mRNA protects mice against immune-mediated inflammatory pathologies. Proc Natl Acad Sci U S A. 2016 Feb 1. pii: 201519906.

Performance of the BG1Luc ER TA method in a qHTS format

February 8, 2016

Ceger P, Allen D, Huang R, Xia M, Casey W. Performance of the BG1Luc ER TA method in a qHTS format. ALTEX. 2015 Jun 28. doi: 10.14573/altex.1505121

Gene Expression Patterns Associated With Histopathology in Toxic Liver Fibrosis

December 14, 2015

Ippolito DL, AbdulHameed MD, Tawa GJ, Baer CE, Permenter MG, McDyre BC, Dennis WE, Boyle MH, Hobbs CA, Streicker MA , Snowden BS, Lewis JA, Wallqvist A, Stallings JD.Gene expression patterns associated with histopathology in toxic liver fibrosis. Toxicol Sci. 2015 Sep 22. pii: kfv214.

Bayesian Integrated Testing Strategy (ITS) for Skin Sensitization Potency Assessment

December 8, 2015

Jaworska JS,· Natsch A, Ryan C, · Strickland J, ·Ashikaga T, · Miyazawa M. 2015. Bayesian integrated testing strategy (ITS) for skin sensitization potency assessment: a decision support system for quantitative weight of evidence and adaptive testing strategy. Arch Toxicol. 2015 Dec; doi: 10.1007/s00204-015-1634-2

Utilizing Whole Slide Images for Pathology Peer Review and Working Groups

November 13, 2015

Malarkey DE, Willson GA, Willson CJ, Adams ET, Olson GR, Witt WM, Elmore SA, Hardisty JF, Boyle MC, Crabbs TA, Miller RA. (2015) Utilizing Whole Slide Images for Pathology Peer Review and Working Groups. Toxicol Pathol. 2015 Oct 27. pii: 0192623315605933

IHC Expression of Cyclin D1, Cytokeratin 20, & Uroplakin III Induced by o-Nitroanisole

October 6, 2015

Cynthia J. Willson, Gordon P. Flake, Robert C. Sills, Grace E. Kissling, and Mark F. Cesta. (2015) Immunohistochemical Expression of Cyclin D1, Cytokeratin 20, and Uroplakin III in Proliferative Urinary Bladder Lesions Induced by o-Nitroanisole in Fischer 344/N Rats. Veterinary Pathology, first published on August 28, 2015 as doi:10.1177/0300985815603432

Integration of metabolic activation w TGx signature to classify genotoxic vs nongenotoxic chemicals

October 6, 2015

J.K. Buick, I. Moffat, A. Williams, C.D. Swartz, L. Recio, D. R. Hyduke, H.H. Li, A.J. Fornace Jr, J. Aubrecht, C.L. Yauk. (2015) Integration of metabolic activation with a predictive toxicogenomics signature to classify genotoxic versus nongenotoxic chemicals in human TK6 cells. Environ Mol Mutagen 56:520–534. doi.org/10.1002/em.21940. PMID: 25733247

A predictive TGx signature to classify genotoxic vs non-genotoxic chemicals in human TK6 cells

October 6, 2015

Andrew Williams, Julie K. Buick, Ivy Moffat, Carol D. Swartz, Leslie Recio, Daniel R. Hyduke, Heng-Hong Li, Albert J. Fornace Jr., Jiri Aubrecht, Carole L. Yauk. (2015) A predictive toxicogenomics signature to classify genotoxic versus non-genotoxic chemicals in human TK6 cells. Data in Brief. doi:10.1016/j.dib.2015.08.013

Impact of Genomics Platform & Statistics on Transcriptional BMD & Selection of Chemical PoD

October 6, 2015

Webster AF, Chepelev N, Gagné R, Kuo B, Recio L, Williams A, Yauk CL. (2015) Impact of Genomics Platform and Statistical Filtering on Transcriptional Benchmark Doses (BMD) and Multiple Approaches for Selection of Chemical Point of Departure (PoD). PLoS One. Aug 27;10(8):e0136764. doi: 10.1371/journal.pone.0136764. PMID: 26313361

TGx & traditional approaches to inform MoA and PoD in health risk assessment of benzo[a]pyrene

October 6, 2015

Moffat I, Chepelev NL, Labib S, Bourdon-Lacombe J, Kuo B, Buick JK, Lemieux F, Williams A, Halappanavar S, Malik AI, Luijten M, Aubrecht J, Hyduke DR, Fornace AJ Jr, Swartz CD, Recio L, Yauk CL. (2015) Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a]pyrene in drinking water. Crit Rev Toxicol. 45(1):1-43. doi: 10.3109/10408444.2014.973934. Review. PMID: 25605026

BrdU treatment to measure hepatocellular proliferation does not mask furan-induced mRNA in mouse

October 6, 2015

Webster AF, Williams A, Recio L, Yauk CL. (2014) Bromodeoxyuridine (BrdU) treatment to measure hepatocellular proliferation does not mask furan-induced gene expression changes in mouse liver. Toxicology. 323:26-31. doi: 10.1016/j.tox.2014.06.002. PMID: 24910943

Mining the archives: a cross-platform analysis of gene expression profiles in archival FFPE tissue

October 6, 2015

Webster AF, Zumbo P, Fostel J, Gandara J, Hester SD, Recio L, Williams A, Wood CE, Yauk CL, Mason CE. (2015) Mining the archives: a cross-platform analysis of gene expression profiles in archival formalin-fixed paraffin-embedded (FFPE) tissue. Toxicol Sci. [Epub ahead of print] PMID: 26361796

Safety assessment and single-dose toxicokinetics of the flavouring agent, myricitrin, in rats

September 21, 2015

Robert R. Maronpot, Mihoko Koyanagi, Jeffrey Davis, Leslie Recio, Dean Marbury, Molly Boyle & Shim-Mo Hayashi. (2015) Safety assessment and single-dose toxicokinetics of the flavouring agent, myricitrin, in Sprague Dawley rats. Food Additives & Contaminants: Part A (2015).

Genotoxicity evaluation of the flavonoid, myricitrin, and its aglycone, myricetin

August 3, 2015

Hobbs CA, Swartz C, Maronpot R, Davis J, Recio L , Koyanagi M, Hayashi SM. (2015) Genotoxicity evaluation of the flavonoid, myricitrin, and its aglycone, myricetin. Food and Chemical Toxicology 83 (2015) 283-292.

Histopathological and Immunohistochemical Characterization of Cell Leisons in Stomach of Rats

July 24, 2015

Janardhan KS , Rebolloso Y, Hurlburt G, Olson D, Lyght O, Clayton NP, Gruebbel M, Picut C, Shackelford C, Herbert RA. (2015) Histopathological and Immunohistochemical Characterization of Methly Eugenol-induced Nonneoplastic and Neoplastic Neuroendocrine Cell Lesions in Glandular Stomach of Rats. Toxicologic Pathology. 2015 Jul.

Hormone Receptor Expression in Spontaneous Uterine Adenocarcinoma in Fischer 344 Rats

July 15, 2015

Willson, C.J., Herbert, R.A., Cline, J.M. (2015) Hormone Receptor Expression in Spontaneous Uterine Adenocarcinoma in Fischer 344 Rats. Toxicologic Pathology. 2015 Jul. 7

JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay

June 1, 2015

Uno, Y., Kojima, H., Omori, T., Corvi, R., Honma, M., Schechtman, L.M., Tice, R.R., Beevers, C., De Boeck, M., Burlinson, B., Hobbs, C.A., Kitamoto, S., Kraynak, A.R., McNamee, J., Nakagawa, Y., Pant, K., Plappert-Helbig, U., Priestley, C., Takasawa, H., Wada, K., Wirnitzer, U., Asano, N., Escobar, P., Lovell, D., Morita, T., Nakajim, M., Ohno, Y., Hayashi, M. (2015) JaCVAM-organized international validation study of the in vivo rodent alkaline comet assay for detection of genotoxic carcinogens: II. Summary of definitive validation study results. Mutation Research. 2015 Apr. 29

Long noncoding RNA lincRNA-p21 is the major mediator of UVB-induced and p53-dependent apoptosis

April 7, 2015

Long noncoding RNA lincRNA-p21 is the major mediator of UVB-induced and p53-dependent apoptosis in keratinocytes.Hall JR, Messenger ZJ, Tam HW, Phillips SL, Recio L, Smart RC. Cell Death Dis. 2015 Mar 19

Differential expression of long noncoding RNAs in the livers of female B6C3F1 mice

April 7, 2015

Differential expression of long noncoding RNAs in the livers of female B6C3F1 mice exposed to the carcinogen furan. Recio L, Phillips SL, Maynor T, Waters M , Jackson AF, Yauk CL. Toxicol Sci. 2013 Oct;135(2):369-79.

Comet assay evaluation of six chemicals of known genotoxic potential in rats

April 1, 2015

Hobbs CA L. Recio L, Streicker M, Boyle M , Tanaka J, Shiga A, Witt KL. Genet. Toxicol. Environ. Mutagen. (2015).

Methyl Isobutyl Ketone Exposure-Related Increases in Specific Measures of Nephropathy in Male Rats

April 1, 2015

Borghoff SJ , Poet TS, Green S, Davis J, Hughes B, Mensing T, Sarang SS, Lynch AM, Hard GC. Toxicology. 2015 Mar 19

Gene Expression of Mesothelioma in Vinylidene Chloride-exposed F344/N Rats Reveal Immune Dysfunction

February 20, 2015

Blackshear PE1, Pandiri AR2, Nagai H3, Bhusari S3, Hong HH3, Ton TV3, Clayton NP3, Wyde M4, Shockley KR5, Peddada SD5, Gerrish KE6, Sills RC3, Hoenerhoff MJ7. Toxicol Pathol. 2014 Jun 23

Predicting chemically-induced skin reactions. Part II: QSAR models of skin permeability

January 20, 2015

Alves VM, Muratov E, Fourches D, Strickland J, Kleinstreuer NC, Andrade CH, Tropsha A. (2015) Predicting chemically-induced skin reactions. Part II: QSAR models of skin permeability and the relationships between skin permeability and skin sensitization. Toxicology and Applied Pharmacology,

Predicting chemically-induced skin reactions. Part I: QSAR models of skin sensitization

January 20, 2015

Alves VM, Muratov E, Fourches D, Strickland J, Kleinstreuer NC, Andrade CH, Tropsha A. (2015) Predicting chemically-induced skin reactions. Part I: QSAR models of skin sensitization and their application to identify potentially hazardous compounds. Toxicology and Applied Pharmacology,

t4 workshop report: Integrated Testing Strategies (ITS) for Safety Assessment ALTEX

December 5, 2014

Rovida C, Alépée N, Api AM, Basketter DA, Bois FY, Caloni F, Corsini E, Daneshian M, Eskes C, Ezendam J, Fuchs H, Hayden P, Hegele-Hartung C, Hoffmann S, Hubesch B, Jacobs MN, Jaworska J, Kleensang A, Kleinstreuer N, Lalko J, Landsiedel R, Lebreux F, Luechtefeld T, Locatelli M, Mehling A, Natsch A, Pitchford JW, Prater D, Prieto P, Schepky A, Schuurmann G, Smirnova L, Toole C, van Vliet E, Weisensee D, Hartung T. 2014. t4 workshop report: Integrated Testing Strategies (ITS) for Safety Assessment ALTEX (in press).

Application of reverse dosimetry to compare in vitro and in vivo estrogen receptor activity

December 5, 2014

Chang X, Kleinstreuer N, Ceger P, Hsieh J-H, Allen D, Casey W. Application of reverse dosimetry to compare in vitro and in vivo estrogen receptor activity. Applied In Vitro Toxicology (in press).

Immediate and long-term consequences of vascular toxicity during zebrafish development

December 5, 2014

Tal TL, McCollum CW, Harris PS, Olin J, Kleinstreuer N, Wood CE, Hans C, Shah S, Merchant FA, Bondesson M, Knudsen TB, Padilla S, Hemmer MJ. 2014. Immediate and long-term consequences of vascular toxicity during zebrafish development. Reprod Toxicol 48:51–61.

Open source software implementation of an integrated testing strategy for skin sensitization potency

December 5, 2014

Pirone JR, Smith M, Kleinstreuer NC, Burns TA, Strickland J, Dancik Y, Morris R, Rinckel LA, Casey W, Jaworska JA. 2014. Open source software implementation of an integrated testing strategy for skin sensitization potency based on a Bayesian network. ALTEX 31: 336–340.

Comments how to make the new vision of toxicity testing in the 21st century a reality

December 5, 2014

McKim A, Goldberg A, Kleinstreuer N, Busquet F, Andersen M. 2014. Roundtable discussion: Comments on how to make the new vision of toxicity testing in the 21st century a reality. Applied In Vitro Toxicology 1(1): 5–10.

t4 workshop report: Lessons Learned, Challenges, and Opportunities: The U.S. EDSP

December 5, 2014

Juberg DR, Borghoff SJ, Becker RA, Casey W, Hartung T, Holsapple MP, Marty MS, Mihaich EM, Van Der Kraak G, Wade MG, Willett CE, Andersen ME, Borgert CJ, Coady KK, Dourson ML, Fowle JR, Gray LE, Lamb JC, Ortego LS, Schug TT, Toole CM, Zorrilla LM, Kroner OL, Patterson J, Rinckel LA, Jones BR. 2014. t4 workshop report: Lessons Learned, Challenges, and Opportunities: The U.S. Endocrine Disruptor Screening Program. ALTEX 31:63-78.

Report on the International Workshop on Alternatives to the Murine Histamine Sensitization Test

December 5, 2014

Isbrucker R, Arciniega J, McFarland R, Chapsal J-M, Xing D, Bache C, Nelson S, Costanzo A, Hoonakker M, Castiaux A, Halder M, Casey W, Johnson N, Jones B, Doelling V, Sprankle C, Rinckel L, Stokes W. 2014. Report on the International Workshop on Alternatives to the Murine Histamine Sensitization Test (HIST) for Acellular Pertussis Vaccines: State of the Science and the Path Forward. Biologicals 42(2):114-122.

Profiling of the Tox21 10K compound library for agonists and antagonists of the estrogen receptor

December 5, 2014

Huang R, Sakamuru S, Martin MT, Reif DM, Judson RS, Houck KA, Casey W, Hsieh J-H, Shockley KR, Ceger P, Fostel J, Witt KL, Tong W, Rotroff DM, Zhao T, Shinn P, Simeonov A, Dix DJ, Austin CP, Kavlock RJ, Tice RR, Xia M. 2014. Profiling of the Tox21 10K compound library for agonists and antagonists of the estrogen receptor alpha signaling pathway. Nature Scientific Reports

Quantitative profiling of environmental chemicals and drugs that modulate farnesoid X receptor

November 5, 2014

Hsu CW, Zhao J, Huang R, Hsieh J-H, Hamm J, Chang X, Houck K, M Xia. 2014. Quantitative high-throughput profiling of environmental chemicals and drugs that modulate farnesoid X receptor. Nature Scientific Reports

Case study on the utility of hepatic gene expression profiling in the risk assessment of furan.

October 15, 2014

Jackson AF, Williams A, Recio L, Waters MD, Lambert IB, Yauk CL. 2014. Case study on the utility of hepatic global gene expression profiling in the risk assessment of the carcinogen furan. Toxicol Appl Pharmacol. 274(1):63-77.

Cytotoxicity of naphthalene toward cells from target and non-target organs in vitro.

October 15, 2014

Kedderis GL, Shepard KG, Recio. L. 2014. Cytotoxicity of naphthalene toward cells from target and non-target organs in vitro. Chem Biol Interact. 209:85-95.

Relative potency for altered humoral immunity induced by polybrominated & polychlorinated dioxins.

October 15, 2014

Frawley R, DeVito M, Walker NJ, Birnbaum L, White K Jr, Smith M, Maynor T, Recio L, Germolec D. 2014. Relative potency for altered humoral immunity induced by polybrominated and polychlorinated dioxins/furans in female B6C3F1/N mice. Toxicol Sci. 139(2):488-500.

BrdU treatment to measure hepatocellular proliferation does not mask furan-induced mRNA changes.

October 15, 2014

Webster AF, Williams A, Recio L, Yauk CL. 2014. Bromodeoxyuridine (BrdU) treatment to measure hepatocellular proliferation does not mask furan-induced gene expression changes in mouse liver. Toxicology. 323C:26-31.

Performance standards and alternative assays: practical insights from skin sensitization.

August 28, 2013

Kolle SN, Basketter DA, Casati S, Stokes WS, Strickland J, van Ravenzwaay B, Vohr HW, and Landsiedel R. 2013. Performance standards and alternative assays: Practical insights from skin sensitization. Regul Toxicol Pharmacol 65: 278-85.

A computational model predicting disruption of blood vessel development.

August 28, 2013

Kleinstreuer N, Dix D, Rountree M, Baker N, Sipes N, Reif D, Spencer R, Knudsen T. (2013) A Computational Model Predicting Disruption of Blood Vessel Development. PLoS Comput Biol 9(4): e1002996.

Meeting Report: Urinary Pathology; Sixth Research Triangle Park Rodent Pathology Course

June 3, 2013

Boyle MC, Boyle MH, and the Sixth Rodent Pathology Course Committee. 2013. Meeting Report: Urinary Pathology; Sixth Research Triangle Park Rodent Pathology Course. Vet. Pathol. 50(3): 563-8.

Preparation of archival formalin-fixed paraffin-embedded mouse liver samples for use with the Agilent gene expression microarray platform.

April 29, 2013

Jackson AF, Williams A, Moffat I, Phillips SL, Recio L, Waters MD, Lambert IB, Yauk CL. 2013. Preparation of archival formalin-fixed paraffin-embedded mouse liver samples for use with the Agilent gene expression microarray platform. J Pharmacol Toxicol Methods. S1056-8719(13)00211-6.

Biomarkers of Exposure and Effect in Human Lymphoblastoid TK6 Cells Following [13C2]-Acetaldehyde Ex

April 29, 2013

Moeller BC, Recio L, Green A, Sun W, Wright FA, Bodnar WM, and Swenberg JA. 2013. Biomarkers of Exposure and Effect in Human Lymphoblastoid TK6 Cells Following [13C2]-Acetaldehyde Exposure. Toxicol Sci. 133(1): 1-12. Editor’s Highlight

A C. elegans Screening Platform for the Rapid Assessment of Chemical Disruption of Germline Function

April 28, 2013

Allard P, Kleinstreuer N, Knudsen T, Colaiacovo M. (2013) A novel chemical screening strategy for assessing environmental germline disruption in C. Elegans. Env Health Persp doi:10.1289/ehp.1206301.

Proceedings of the 2012 National Toxicology Program Satellite Symposium.

April 9, 2013

Elmore S.A., Berridge, B. R., Boyle, M.C., Cora, M.C., Hoenerhoff, M.J., Kooistra, L., Laast, V.A., Morrison, J.P., Rao, D.,Rinke, M. and Yoshizawa, K. 2013. Proceedings of the 2012 National Toxicology Program Satellite Symposium. Toxicol Pathol. 41: 151-180.

Evaluation of the Association between Maternal Smoking, Childhood Obesity, and Metabolic Disorders:

April 9, 2013

Behl, M., Rao, D., Aagaard, K., Davidson, T.L., Levin, E.D., Slotkin, T.A., Srinivasan, S., Wallinga, D., White, M.F., Walker,V.R., Thayer, K.A., Holloway, A.C. 2013. Evaluation of the Association between Maternal Smoking, Childhood Obesity, and Metabolic Disorders: A National Toxicology Program Workshop Review. Environ Health Perspect. 121(2): 170-180.

Evaluation of 90-day oral rat toxicity studies on the food additive, gum ghatti

December 12, 2012

Maronpot, R., Davis, J., Moser, G., Giri, D., Hayashi, S. 2012. Evaluation of 90-day oral rat toxicity studies on the food additive, gum ghatti. Food and Chemical Toxicology, 51: 215-224.

Report on the International Workshop on Alternative Methods for Human & Veterinary Rabies Vaccines

August 14, 2012

Stokes W, McFarland R, Kulpa-Eddy J, Gatewood D, Levis R, Halder M, Pulle G, Kojima H, Casey W, Gaydamaka A, Miller T, Brown K, Lewis C, Chapsal, Bruckner L, Gairola S, Kamphuis E, Rupprecht CE, Wunderli P, McElhinney L, De Mattia F, Gamoh K, Hill R, Reed D, Doelling V, Johnson N, Allen D, Rinckel L, and Jones B. 2012. Report on the International Workshop on Alternative Methods for Human and Veterinary Rabies Vaccine Testing: State of the Science and Planning the Way Forward. Biologicals.

Role of Estrogen Receptor Signaling Required for Endometriosis-like lesion Establishment in mouse

July 19, 2012

Burns, KA, Rodriguez, KF, Hewitt, SC, Janardhan, KS, Young, SL, Korach, KS. Role of Estrogen Receptor Signaling Required for Endometriosis-like lesion Establishment in a mouse model. Endocrinology Epub June 14 2012.

Proliferative and Non-proliferative Lesions of the Rat and Mouse Central & Peripheral Nervous System

July 19, 2012

Kaufmann,W., Bolon, B., Bradley, A., Butt, M., Czasch, S., Garman, R.H., George, C., Groters S., Krinke, G., Little, P., McKay, J., Narama, I., Rao, D., Shibutani, M., and Sills, R.A. (2012) Proliferative and Non-proliferative Lesions of the Rat and Mouse Central and Peripheral Nervous Systems. Toxicol Pathol.40: 87S-157S.

Proceedings of the 2011 National Toxicology Program Satellite Symposium

July 19, 2012

Boorman,G., Crabbs, T.A., Kolenda-Roberts, H., Latimer, K., Miller, a.D., Muravnick, K.B., Nyska, A., Ochoa, r., Pardo, I.d., Ramot, Y., Rao, D.B., Schuh, J., Suttie, A., Travlos, G.S., Ward, J.M., Wolf, J.C., and Elmore, S.A. Proceedings of the 2011 National Toxicology Program Satellite Symposium. Toxicol Pathol. (2012) 40: 321-344.

Bronchial and bronchiolar fibrosis in rats exposed to 2,3-pentanedione vapors

July 19, 2012

Morgan DL, Jokinen MP, Price HC, Gwinn WM, Palmer SM, Flake GP. Bronchial and bronchiolar fibrosis in rats exposed to 2,3-pentanedione vapors: Implication for bronchiolitis obliterans in humans. Toxicol. Pathol. 40:448-465, 2012.

Morphologic aspects of rodent cardiotoxicity in a restrospective evaluation of National Toxicology P

July 19, 2012

Jokinen MP, Lieuallen WG, Boyle MC, Johnson CL, Malarkey DE, Nyska A. Morphologic aspects of rodent cardiotoxicity in a restrospective evaluation of National Toxicology Program studies. Toxicol. Pathol. 39:850-860, 2011.

Intestinal Lymphangiectasis and Lipidosis in Rats Following Subchronic Exposure to Indole-3-Carbinol

July 19, 2012

Michael C. Boyle, Torrie A. Crabbs, Michael E. Wyde, J. Todd Painter, Georgette D. Hill, David E. Malarkey, Warren G. Lieuallen, and Abraham Nyska(2012) Intestinal Lymphangiectasis and Lipidosis in Rats Following Subchronic Exposure to Indole-3-Carbinol via Oral Gavage. Toxicologic Pathology, Vol. 40, No. 4, 561–576 (2012).

Comparison Comet Assay Dose-Response Ethyl Methanesulfonate Freshly Prepared Vs Cryoprepared Tissues

July 18, 2012

Recio, L., Kissling, G.E., Hobbs, C.A., and Witt, K.L. (2012) Comparison of Comet Assay Dose-Response for Ethyl Methanesulfonate Using Freshly Prepared Versus Cryopreserved Tissues. Environmental and Molecular Mutagenesis 53, 101-113. DOI: 10.1002/em.20694.

Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti

June 18, 2012

Hobbs, C.A., Swartz, C., Maronpot, R., Davis, J., Recio, L., and Hayashi, S. (2012) Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti. Food and Chemical Toxicology 50, 854-860. DOI: 10.1016/j.fct.2011.11.021.

Genotoxicity of Styrene-Acrylonitrile Trimer in Brain, Liver, and Blood Cells of Weanling F344 Rats

June 18, 2012

Hobbs, C.A., Chhabra, R.S., Recio, L., Streicker, M. and Witt, K.L. (2012) Genotoxicity of Styrene-Acrylonitrile Trimer in Brain, Liver, and Blood Cells of Weanling F344 Rats. Environmental and Molecular Mutagenesis 53, 227-238. DOI: 10.1002/em.21680

Assessment of Naphthalene-Induced Genotoxicity & Gluathione Detoxication in Human TK6 Lymphoblasts

March 26, 2012

Recio L, Shepard KG, Hernandez, LG, Kedderis GL. 2012. Dose-Response Assessment of Naphthalene-Induced Genotoxicity and Glutahione Detoxication in Human TK6 Lymphoblasts. Toxicol. Sci. 126(2):405-412.

Therapeutic Ultrasound as a Potential Male Contraceptive

March 23, 2012

Tsuruta JK, Dayton PA, Gallippi CM, O’Rand MG, Streicker MA, Gessner, RC, Gregory TS, Silva E JR, Hamil, KG, Moser GJ, Sokai, DC. 2012. Therapeutic Ultrasound as a Potential Male Contraceptive: Power, Frequency and Temperature Required to Deplete Rat Testes of Meiotic Cells and Epididymides of Sperm Determined Using Commercially Available System. Reproductive Biology and Endocrinology 10:7.

15-Day Intact Male Rat Assay: Evaluation of an Antithyroid Chemical and a Negative Control Chemical

March 23, 2012

Becker RA, Bergfelt, DR, Borghoff S, Davis JP, Hamby BT, O’Connor JC, Kaplan AM, Sloan CS, Tyl, RW, Wade M, Marty MS. 2012. Interlaboratory Study Comparison of the 15-Day Intact Adult Male Rat Screening Assay: Evaluation of an Antithyroid Chemical and a Negative Control Chemical. Birth Defects Research (Part B). (95):63-78.

Improving Animal Welfare and Reducing Animal Use for Veterinary Vaccine Potency Testing

January 23, 2012

Stokes WS, Brown K, Kulpa-Eddy J, Srinivas G, Halder M, Draayer H, Galvin J, Claasen I, Gifford G, Woodland R, Doelling V, Jones B. 2011. Improving Animal Welfare and Reducing Animal Use for Veterinary Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):84-105.

Alt. Methods to Reduce, Refine & Replace Animal Use for Veterinary Vaccine Post-Licensing Testing

January 13, 2012

Kulpa-Eddy J, Srinivas G, Halder M, Brown K, Draayer H, Glavin J, Claassen I, Gifford G, Woodland R, Doelling V, Jones, B, Stokes WS. 2011. Alternative Methods and Strategices to Reduce, Refine and Replace Animal Use for Veterinary Vaccine Post-Licensing Safety Testing: State of the The Science and Future Directions.Procedia in Vaccinology(5):106-119.

Alternative Methods to Reduce, Refine & Replace Animal Use for Human Vaccine Post-Licensing Testing

January 13, 2012

Isbrucker R, Levis R, Casey W, McFarland R, Schmitt M, Arciniega J, Descamps J, Finn T, Hendriksen C, Horiuchi Y, Keller J, Kojima H, Sesardic D, Stickings P, Johnson NW, Allen D. 2011. Alternative Methods and Strategies to Reduce, Refine and Replace Animal Use for Human Vaccine Post-Licensing Safety Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):47-59.

Non-Animal Replacement Methods for Veterinary Vaccine Potency Testing

January 13, 2012

Kulp-Eddy J, Srinivas G, Halder M, Hill R, Brown K, Roth J, Draayer H, Galvin G, Woodland R, Doelling V, Jones B, Stokes WS. 2011. Non-Animal Replacement Methods for Veterinary Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):60-83.

Improving Animal Welfare and Reducing Animal Use for Human Vaccine Potency Testing

January 13, 2012

Casey W, Schmitt M, McFarland R, Isbrucker R, Levis R, Arciniega J, Descamps J, Finn T, Hendriksen C, Horluchi Y, Keller, J, Kojima H, Sesardic D, Stickings P, Johnson NW, Lipscomb E, Allen D. 2011. Improving Animal Welfare Reducing Animal Use for Human Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5):33-46.

Non-Animal Replacement Methods for Human Potency Testing: State of the Science and Future Directions

January 13, 2012

McFarland R, Verthelyi D, Casey W, Arciniega J, Isbrucker R, Schmitt M, Finn T, Descamps J, Horiuchi Y, Sesardic D, Stickings P, Johnson NW, Lipscomb E, Allen D. 2011. Non-Animal Replacement Methods for Human Vaccine Potency Testing: State of the Science and Future Directions. Procedia in Vaccinology(5): 16-32.

Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti

December 21, 2011

Hobbs CA, Swartz C, Maronpot R, Davis J, Recio L, and Hayashi S. 2011. Evaluation of the Genotoxicity of the Food Additive, Gum Ghatti. Food and Chemical Toxicology DOI:10.1016/j.fct.2011.11.021.

Improvement of In Vivo Genotoxicity: Acute Tests and Integration into Standard Toxicity Testing

November 26, 2011

Rothfuss A, Honma M, Czich A, Aardema MJ, Burlinson B, Galloway S, Hamada S, Kirkland D, Heflich RH, Howe J, Nakajima M, O’Donovan M, Plappert-Helbig U, Priestley C, Recio L, Schuler M, Uno Y, and Martus HJ. 2011. Improvement of In Vivo Genotoxicity: Combination of Acute Tests and Integration into Standard Toxicity Testing. Mutat. Res. 723: 108-120.

Comet Assay Dose-Response for Ethyl Methanesulfonate Using Freshly Prepared Vs. Cryopreserved Tissue

November 26, 2011

Recio L, Kissling GE, Hobbs CA, and Witt KL, 2011. Comparison of Comet Assay Dose-Response for Ethyl Methansulfonate Using Freshly Prepared Versus Cryopreserved Tissues. Environmental and Molecular Mutagenesis (in press).

Using Fewer Animals to Identify Chemical Eye Hazards: Criteria to Maintain Hazard Classification

October 20, 2011

Haseman JK,Allen DG,Lipscomb EA,Truax JF,Stokes WS. 2011. Using Fewer Animals to Identify Chemical Eye Hazards: Revised Criteria Necessary to Maintain Equivalent Hazard Classification. Regulatory Toxicology and Pharmacology 61:98-104.

Disseminiated Histoplasmosis with Concurrent Oral Candidiasis in an Eclectus Parrot

October 19, 2011

Quist EM,Belcher C,Levine G,Johnson M,Heatley JJ,Kiupel M, Giri D. 2011. Disseminated Histoplasmosis with Concurrent Oral Candidiasis in an Electus Parrot (Eclectus Roratus). Avian Pathol. 40(3):339.

Cerebral and Renal Phaeohyphomycosis in a Dog Infected with Bipolaris Species

October 19, 2011

Giri DK,Sims WP,Sura R,Cooper JJ,Gavrilov BK,Mansell J. 2011. Cerebral and Renal Phaeohhyphomycosis in a Dog Infected with Bipolaris Species. Vet Pathol. 48(3):754-7.

Neoplastic and Nonneoplastic Liver Lesions Induced by Dimethylnitrosamine in Japanese Medaka Fish

October 17, 2011

Hobbie KR,DeAngelo AB,George MH,Law JM. 2011. Neoplastic and Nonneoplastic Liver Lesion Induced by Dimethylnitrosamine in Japanes Medaka Fish. Vet. Pathol. Online. DOI: 10.1177/0300985811409443.

Liver Toxicity and Carcinogenicity in f344/n Rats and b6cf1 Mice Exposed to Kava Kava

September 22, 2011

Behl M,Nyska A,Chhabra RS,Travlos G,Fomby LM,Sparrow BR,Hejtmancik MR,Chan PC. 2011. Liver Toxicity and Carcinogenicity in f344/n Rats and b6c3f1 Mice Exposed to Kava Kava. Food and Chemical Toxicology. In press for publication in November 2011.

Safety Assessment of Allergic Contact Dermatitis Hazards: An Analysis Supporting Reduced Animal Use

September 22, 2011

Haseman JK,Strickland J,Allen D,Salicru E,Paris M,Tice RR,Stokes WS. 2011. Safety Assessment of Allergic Contact Dermatitis Hazards: An Analysis Supporting Reduced Animal Use for the Murine Local Lymph Node Assay. Regulatory Toxicology and Pharmacology 59: 191-96.

Assessment of a Regulated AAV2 Gene Transfer Vector After Murine Submandibular Glands Delivery

September 21, 2011

Zheng C,Voutetakis A,Goldstein B,Afione S,Rivera V,Clackson T,Wenk M,Boyle M,Nyska A,Chiorini J,Vallant M,Irwin R,Baum B. 2011. Assessment of the Safety and Biodistribution of a Regulated AAV2 Gene Transfer Vector After Delivery to Murine Submandibular Glands. Toxicological Sciences.

Effects of Atrazine on Steroidgenesis in Rat Granulosa and H295R Adrenal Cortical Carcinoma Cells

September 20, 2011

Tinfo NS,Hotchkiss MG,Buckalew AR,Zorrilla LM,Cooper RL,Laws SC. 2011. Understanding the Effects of Atrazine on Steriodogenesis in Rat Granulosa and H295 Adrenal Cortical Carcinoma Cells.Reprod. Toxicol. 31(2):184-93

Histopathological Evaluation of the Nervous System in National Toxicology Program of Rodent Studies

September 18, 2011

Rao, D, Little, PB, Malarkey, D.E, Herbert, RA, Sills, RC. 2011. Histopathological Evaluation of the Nervous System in National Toxicology Program of Rodent Studies: A Modified Approach. Toxicologic Pathology 39: 463-470.

Genetic Toxicology in the 21st Century: Reflections and Future Directions

April 28, 2011

Mahadevan B, Snyder R, Waters M, Benz RD, Kemper RA, Tice RR and Richard AM. 2011. Genetic Toxicology in the 21st Century: Reflections and Future Directions. Environ. Mol. Mutagen. 52.

Effects of Hormonally Active Agents on Steroid Hormone Receptor Expression and Cell Proliferation

March 16, 2011

Hill GD, Moore AB, Kissling GE, Flagler ND, Ney E, Cline JM, Dixon D. 2011. Effects of Hormonally Active Agents on Steroid Hormone Receptor Expression and Cell Proliferation in the Myometrium of Ovariectomized Macaques. Toxicol. Pathol. 39(3): 508-515.

Toxicity Study of Green Tea Extract in Rats and Mice

December 1, 2010

Chan PC, Ramot Y, Malarkey DE, Blackshear P, Kissling GE, Travlos G, Nyska A. 2010. Fourteen week Toxicity Study of Green Tea Extract in Rats and Mice. Toxicol. Pathol. 38(7): 1070-1084.

Characterizing and Predicting Carcinogenicity and Mode of Action

April 24, 2010

Waters M, Jackson MA, and Lea IA. 2010. Characterizing and predicting carcinogenicity and mode of action using conventional and toxicogenomics methods. Mutat Res 705: 184-200.

Dose-Response of Four Genotoxic Chemicals in a Combined Mouse and Rat Micronucleus and Comet Assay

April 1, 2010

Recio L, Hobbs CA, Caspary W, Witt KL. 2010. Dose-Response Assessment of Four Genotoxic Chemicals in a Combined Mouse and Rat Micronucleus and Comet Assay Protocol. Toxicol Sciences 35(2): 149-162.

No Biomarkers of Genetic Damage or Pathological Changes in Male Rats Administered Ritalin

January 1, 2010

Witt KL, Malarkey DE, Hobbs CA, Davis JP, Kissling GE, Caspary W, Travlos G, Recio L. 2010. No increases in biomarkers of genetic damage or pathological changes in heart and brain tissues in male rats administered methylphenidate hydrochloride (Ritalin) for 28 days. Environ Mol Mutagen 51(1): 80-88.